COVID-19 RT-PCR test Controls – Positive, Negative, and Internal
Negative (no template control) – negative for all targets detected (Ct Not Detected)
Positive – positive for all targets detected (Ct < 38)
Internal extraction (Hs_RPP30) – negative for SARS-CoV-2 targets (Ct Not Detected), positive for RNase P (RP) target (Ct < 40)
Negative extraction (NEC) – negative for SARS-CoV-2 targets (Ct Not Detected), positive for RNase P (RP) target (Ct < 40)
If any control does not perform as described above, run is considered invalid and all specimens are repeated from extraction step.